plexin b2 apc Search Results


plexin b2 apc  (R&D Systems)
92
R&D Systems plexin b2 apc
a Flow panel showing the gating of PLXNB2 + and PLXNB2 - cells sorted from dissociated primary TN3 L2G + PDX cells for clustering in ( b , c ). b , c Representative images of clustering ( b ) and average cluster area and cluster count curves of sorted PLXNB2 high and low TN3 PDX cells ( c ); n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. d , e Representative images ( d ) and cluster area ( e ) of MDA-MB-231 cells transfected with siRNA control (siCon), PLXNB2 SmartPool siRNA ( siPLXNB2 ), and single siRNA ( siPLXNB2-10 ), n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. f , g Representative images ( f ) and cluster area ( g ) of MDA-MB-468 cells transfected with siCon, si PLXNB2 , and si PLXNB2 -10, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. h Schematic of PLXNB2 domains: Extracellular (Ecto) domains include SEMA = SEMAphorin domain; IPT = Ig-like fold domain; and PSI = <t>Plexin-SEMAphorin-integrin</t> domain. Intracellular domains include RBD = Rho-binding domain; GAP = GTPase activating protein domain; and VTDL = PDZ-domain binding site (Rho-GEF binding). i Immunoblots of overexpressed PB2 mutants with either full-length (fl) or truncated (tr) depletions: mutated RBD (mRBD) tr, depleted Ecto domain (dECTO) fl and depleted VTDL (dVTDL) tr in MDA-MB-231 PB2 KO cells. j , k Representative images ( j ) and cluster area growth curves ( k ) of MDA-MB-231 PB2 KO clusters with overexpression of PLXNB2 full-length or mutants (mRBD, dECTO, or dVTDL); Con vs. KO + PLXNB2 p = 0.67, Con vs. KO1 p = 0.0002, Con vs. KO2 p = 1.43e-6, KO + PLXNB2 vs. KO+mRBD p = 0.04, KO + PLXNB2 vs. KO+dECTO p = 1.69e-6, KO + PB2 vs. KO+dVTDL p = 0.005, KO + PLXNB2 vs. KO1 p = 0.0002, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. l – o Bioluminescence images ( l , n ) and quantified BLI signals ( m , o ) of dissected lungs ex vivo after transfections with siCon, siPLXNB2 ( l ), or si PLXNB2 -11 ( n ), n = 4 mice/group ( l , m ) and n = 3 mice/group ( n , o ). P-value between two groups was calculated using two-sided unpaired t-test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.
Plexin B2 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
plexin b2 apc - by Bioz Stars, 2026-05
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plxnb1 mouse igg2a n a apc r d systems  (R&D Systems)
93
R&D Systems plxnb1 mouse igg2a n a apc r d systems
Antibodies used to test enrichment of latently infected cells from mixed cell populations.
Plxnb1 Mouse Igg2a N A Apc R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plxnb1 mouse igg2a n a apc r d systems/product/R&D Systems
Average 93 stars, based on 1 article reviews
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plexin-b2 antibody, anti-human, apc, reafinity  (Miltenyi Biotec)
N/A
Identification and enumeration of plexin-B2+ cells by flow cytometry
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plexin-b2 antibody, anti-mouse, apc, reafinity  (Miltenyi Biotec)
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Identification and enumeration of plexin-B2+ cells by flow cytometry
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Image Search Results


a Flow panel showing the gating of PLXNB2 + and PLXNB2 - cells sorted from dissociated primary TN3 L2G + PDX cells for clustering in ( b , c ). b , c Representative images of clustering ( b ) and average cluster area and cluster count curves of sorted PLXNB2 high and low TN3 PDX cells ( c ); n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. d , e Representative images ( d ) and cluster area ( e ) of MDA-MB-231 cells transfected with siRNA control (siCon), PLXNB2 SmartPool siRNA ( siPLXNB2 ), and single siRNA ( siPLXNB2-10 ), n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. f , g Representative images ( f ) and cluster area ( g ) of MDA-MB-468 cells transfected with siCon, si PLXNB2 , and si PLXNB2 -10, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. h Schematic of PLXNB2 domains: Extracellular (Ecto) domains include SEMA = SEMAphorin domain; IPT = Ig-like fold domain; and PSI = Plexin-SEMAphorin-integrin domain. Intracellular domains include RBD = Rho-binding domain; GAP = GTPase activating protein domain; and VTDL = PDZ-domain binding site (Rho-GEF binding). i Immunoblots of overexpressed PB2 mutants with either full-length (fl) or truncated (tr) depletions: mutated RBD (mRBD) tr, depleted Ecto domain (dECTO) fl and depleted VTDL (dVTDL) tr in MDA-MB-231 PB2 KO cells. j , k Representative images ( j ) and cluster area growth curves ( k ) of MDA-MB-231 PB2 KO clusters with overexpression of PLXNB2 full-length or mutants (mRBD, dECTO, or dVTDL); Con vs. KO + PLXNB2 p = 0.67, Con vs. KO1 p = 0.0002, Con vs. KO2 p = 1.43e-6, KO + PLXNB2 vs. KO+mRBD p = 0.04, KO + PLXNB2 vs. KO+dECTO p = 1.69e-6, KO + PB2 vs. KO+dVTDL p = 0.005, KO + PLXNB2 vs. KO1 p = 0.0002, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. l – o Bioluminescence images ( l , n ) and quantified BLI signals ( m , o ) of dissected lungs ex vivo after transfections with siCon, siPLXNB2 ( l ), or si PLXNB2 -11 ( n ), n = 4 mice/group ( l , m ) and n = 3 mice/group ( n , o ). P-value between two groups was calculated using two-sided unpaired t-test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Computational ranking identifies Plexin-B2 in circulating tumor cell clustering with monocytes in breast cancer metastasis

doi: 10.1038/s41467-025-62862-z

Figure Lengend Snippet: a Flow panel showing the gating of PLXNB2 + and PLXNB2 - cells sorted from dissociated primary TN3 L2G + PDX cells for clustering in ( b , c ). b , c Representative images of clustering ( b ) and average cluster area and cluster count curves of sorted PLXNB2 high and low TN3 PDX cells ( c ); n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. d , e Representative images ( d ) and cluster area ( e ) of MDA-MB-231 cells transfected with siRNA control (siCon), PLXNB2 SmartPool siRNA ( siPLXNB2 ), and single siRNA ( siPLXNB2-10 ), n = 5 technical replicates examined over 3 independent experiments. P-value was calculated using two-sided unpaired t-test. f , g Representative images ( f ) and cluster area ( g ) of MDA-MB-468 cells transfected with siCon, si PLXNB2 , and si PLXNB2 -10, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. h Schematic of PLXNB2 domains: Extracellular (Ecto) domains include SEMA = SEMAphorin domain; IPT = Ig-like fold domain; and PSI = Plexin-SEMAphorin-integrin domain. Intracellular domains include RBD = Rho-binding domain; GAP = GTPase activating protein domain; and VTDL = PDZ-domain binding site (Rho-GEF binding). i Immunoblots of overexpressed PB2 mutants with either full-length (fl) or truncated (tr) depletions: mutated RBD (mRBD) tr, depleted Ecto domain (dECTO) fl and depleted VTDL (dVTDL) tr in MDA-MB-231 PB2 KO cells. j , k Representative images ( j ) and cluster area growth curves ( k ) of MDA-MB-231 PB2 KO clusters with overexpression of PLXNB2 full-length or mutants (mRBD, dECTO, or dVTDL); Con vs. KO + PLXNB2 p = 0.67, Con vs. KO1 p = 0.0002, Con vs. KO2 p = 1.43e-6, KO + PLXNB2 vs. KO+mRBD p = 0.04, KO + PLXNB2 vs. KO+dECTO p = 1.69e-6, KO + PB2 vs. KO+dVTDL p = 0.005, KO + PLXNB2 vs. KO1 p = 0.0002, n = 5 technical replicates examined over 3 independent experiments. P values were calculated using one-sided ANOVA. l – o Bioluminescence images ( l , n ) and quantified BLI signals ( m , o ) of dissected lungs ex vivo after transfections with siCon, siPLXNB2 ( l ), or si PLXNB2 -11 ( n ), n = 4 mice/group ( l , m ) and n = 3 mice/group ( n , o ). P-value between two groups was calculated using two-sided unpaired t-test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

Article Snippet: They were blocked with mouse IgG (Sigma #I5381) for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: Plexin B2 PE (phycoerythrin) (1:1,000, human, R&D Systems #FAB53291P), Plexin B2 APC (1:1,000, human, R&D Systems #FAB53291A), Plexin B2 FITC (1:1,000, mouse, R&D Systems #FAB6836G), CD45 (1:1,000, human, BD Bioscience #557748), EpCAM FITC (1:1,000, human, BD Bioscience #347197), SEMA4A PE (1:1,000, human/mouse, BioLegend #148404), SEMA4A APC (1:1,000, human/mouse Biolegend #148406).

Techniques: Transfection, Control, Binding Assay, Western Blot, Over Expression, Ex Vivo

Antibodies used to test enrichment of latently infected cells from mixed cell populations.

Journal: Virology

Article Title: Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency

doi: 10.1016/j.virol.2022.06.003

Figure Lengend Snippet: Antibodies used to test enrichment of latently infected cells from mixed cell populations.

Article Snippet: Antibody Isotype Secondary a Conjugate Manufacturer (cat. #) CEACAM1 Mouse IgG2b N/A PE R&D Systems (FAB2244P) PLXNB1 Mouse IgG2a N/A APC R&D Systems (FAB53291A) STX16 Rabbit IgG Goat anti-Rabbi APC Thermofisher Scientific (PA5-48340) LIMK1 Mouse IgG2b Goat anti-Mouse PE Novus Biologicals (NBP2-00748) LAX1 Rabbit IgG Goat anti-Rabbit APC Abcam (ab133759) ANXA2 Rabbit IgG N/A PE Abcam (ab210729) LGALS1 Mouse IgG1 Goat anti-Mouse PE Thermofisher Scientific (43–7400) EPHA2 Mouse IgG2a N/A APC R&D Systems (FAB3035) CD80 Mouse IgG1 N/A FITC R&D Systems (FAB140F-025) CCL5 Mouse IgG1 N/A APC R&D Systems (IC278A) MX1 Rabbit IgG Goat anti-Rabbit APC Abcam (ab207414) KRAS Mouse IgG2b Goat anti-Mouse PE Novus Biologicals (NBP2-59413) Open in a separate window CEACAM1 , CEA cell adhesion molecule 1; PLXNB2 , plexin B2; STX16 , syntaxin 16; LIMK1 , LIM domain kinase 1; LAX1 , lymphocyte transmembrane adaptor 1; ANXA2 , annexin A2; LGALS1 , galectin 1; EPHA2 , EPH receptor A2; CD80 , CD80 molecule; CCL5 , C─C motif chemokine ligand 5; MX1 , MX dynamin like GTPase 1; KRAS , KRAS proto-oncogene, GTPase; PE, phycoerythrin; APC, allophycocyanin; FITC, fluorescein isothiocyanate; cat. #, catalog number; N/A, not applicable. a Secondary antibody is not applicable for primary antibodies conjugated to fluorophores.

Techniques: Infection